Tissue Culture, Have You Had Success?

J.E. Shields jshields@indy.net
Fri, 10 Sep 2010 05:37:35 PDT
Setting it up in your kitchen is not all that hard to do, but getting a 
sterile environment is really tricky and, especially for a beginner to test 
tube or flask culture, will take some learning.  It would help to at least 
have taken a microbiology lab course at some time.

We ran into a further problem -- the storage area under lights had too low 
a relative humidity and most of the dished eventually dried out.  The guy I 
was working with had plenty of experience, but we were doing it in his 
basement.  Retired folks have problems like that.

I'm still trying to decide whether I want to try some of it myself or 
not.  I did pick up a used glove box for a sterile transfer 
environment.  Glove boxes are easier to get and keep sterile, but a lot 
less easy to work in than a laminar flow hood.

I prefer twin  scaling or bulb chipping.  I've done this with Lycoris and 
with Haemanthus in the past.  My yields were not much above 1 (one 
surviving bulblet per bulb cut up).  There are apparently some tricks of 
technique to be learned even in simple twin-scaling.  It looked really easy 
when I saw it at Hadeco in South Africa.

In any case, practice on bulbs you can afford to lose!

Jim Shields

At 05:19 PM 9/9/2010 -0700, you wrote:
>This is a very interesting topic, how many of you have tried this and been
>successful?  Honestly, I wouldn't have ever assumed it to be succesful 
>of a sterile lab.

Jim Shields             USDA Zone 5             Shields Gardens, Ltd.
P.O. Box 92              WWW:    http://www.shieldsgardens.com/
Westfield, Indiana 46074, USA
Tel. ++1-317-867-3344     or      toll-free 1-866-449-3344 in USA

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