Tissue culture

Tony Avent Tony@plantdelights.com
Mon, 08 Jul 2013 03:06:12 PDT
Aad:

Crinum are better in tissue culture using liquid media as opposed to typical agar based media.  Liquid media requires constant turning, so is quite a bit more complex for the home gardener.

Tony Avent
Plant Delights Nursery @
Juniper Level Botanic Garden
9241 Sauls Road
Raleigh, North Carolina  27603  USA
Minimum Winter Temps 0-5 F
Maximum Summer Temps 95-105F
USDA Hardiness Zone 7b
email tony@plantdelights.com
website  http://www.plantdelights.com/
phone 919 772-4794
fax  919 772-4752
"I consider every plant hardy until I have killed it myself...at least three times" - Avent
-----Original Message-----
From: pbs-bounces@lists.ibiblio.org [mailto:pbs-bounces@lists.ibiblio.org] On Behalf Of Aad van Beek
Sent: Monday, July 08, 2013 5:33 AM
To: Pacific Bulb Society
Subject: [pbs] Tissue culture

A couple of months ago I came across an site how to do tissue culture at home. (http://kitchenculturekit.com/StiffAffordablePTCfor…).
Didn't do anything with it then. But, based on Tony remark crinums are easy in tissue culture, I'm curious if anybody has some experience with it at home.

Aad,
Sunny and warm Holland 

> From: Tony@plantdelights.com
> To: pbs@lists.ibiblio.org
> Date: Sun, 7 Jul 2013 20:10:40 -0400
> Subject: Re: [pbs] Crinum bulbispermum - to off set or ?
> 
> Steve:
> 
> Yes, crinum are pretty easy in tissue culture.
> 
> Tony Avent
> Plant Delights Nursery @
> Juniper Level Botanic Garden
> 9241 Sauls Road
> Raleigh, North Carolina  27603  USA
> Minimum Winter Temps 0-5 F
> Maximum Summer Temps 95-105F
> USDA Hardiness Zone 7b
> email tony@plantdelights.com
> website  http://www.plantdelights.com/
> phone 919 772-4794
> fax  919 772-4752
> "I consider every plant hardy until I have killed it myself...at least three times" - Avent
> 
> -----Original Message-----
> From: pbs-bounces@lists.ibiblio.org [mailto:pbs-bounces@lists.ibiblio.org] On Behalf Of Steven
> Sent: Sunday, July 07, 2013 4:55 AM
> To: Pacific Bulb Society
> Subject: Re: [pbs] Crinum bulbispermum - to off set or ?
> 
> I have grown lots of Crinum bulbispermum from seed, I've found in my conditions they grow very fast. I have a number of mature specimens in a hot dry North Western aspect where they cop brutal seasonal winds scorching ground baking heat in summer & into the minor zeros for short bursts in winter, they are in poor shale type soil over solid rock, improved by tilling well composted green waste below surface level to about 6" deep.. I too have only ever had a couple of pups out of about 15 very healthy mature plants.. Seed is readily available online, very affordable, fast growing & virus free. Seed or plants are definitely the easiest way to get more flowers in your garden. 
> It would also be interesting to know if they are able to be tissue cultured ??? 
> I would love to reproduce some of the smaller hybridized colours I was given.
> 
> Steven :  )
> Queensland Australia 
> 
> On 07/07/2013, at 7:09 AM, James Waddick <jwaddick@kc.rr.com> wrote:
> 
> >    
> > On Jul 5, 2013, at 7:41 PM, Tony Avent wrote:
> >> 
> >> 
> >> Since according to Dave Lehmiller, true Crinum bulbispemum never offsets, so the best viable option is seed. 
> > 
> > Dear Tony and all, 
> > 
> > 
> >    Today I went out in the 90+ degree sun and observed my original decade plus planting of C. bulbispermum 'Jumbo'. One plant has a single stem without offset, but 2 others have 2 offsets each.  
> > 
> >    Seedlings from these have no or a couple offsets each.  None of them form dense clumps like xpowellii. 
> > 
> >    They look VERY similar in all physical aspects.        Best        Jim
> > 
> > 
> > _______________________________________________
> > pbs mailing list
> > pbs@lists.ibiblio.org
> > http://pacificbulbsociety.org/list.php
> > http://pacificbulbsociety.org/pbswiki/
> _______________________________________________
> pbs mailing list
> pbs@lists.ibiblio.org
> http://pacificbulbsociety.org/list.php
> http://pacificbulbsociety.org/pbswiki/
> _______________________________________________
> pbs mailing list
> pbs@lists.ibiblio.org
> http://pacificbulbsociety.org/list.php
> http://pacificbulbsociety.org/pbswiki/
 		 	   		  






More information about the pbs mailing list