Hi Ernie Thank you very much for your helpful information , yes in long time micropropagation that use callus culture we can wait for mutations , not all will be dominant in resulting plants and we can wait for more variants if our plants can set seeds , sadly this lily is not very fertile and is not self fertile . anyway Good Luck Mohammad Sadegh Ardabil ,Iran On Tuesday, July 8, 2014 12:56:19 AM, Ernie DeMarie via pbs <pbs@lists.ibiblio.org> wrote: Hi Mohammad, Interesting, it does seem that some tissue culture methods can cause changes in the resultant plants. When I did my thesis work many years ago on tissue culturing pelargonium species, I found that using callus cultures to rescue hybrid embryos between different species would sometimes yield both diploid (expected) and tetraploid plants. Perhaps the transformation of the embryos into callus and back to differentiated shoots allowed for some tetraploid cells to form and grow as shoots whereas if the embryo was grown directly into a plant such a cell might not have a chance to become a shoot on its own. I also think that more time in tissue culture means more chance for genetic mutations, so you could get odd plants every now and then from the mutated cell. For example, some Venus Fly Trap (Dionaea muscipula) cultivars apparently arose as tissue cultured mutations. Ernie DeMarie Briarcliff Manor, New York Where rhodohypoxis, hypoxis, many oxalis, ledebourias and drimias are blooming in pots, Zantedeschia blooming in pots and Z albomaculata in the garden after surviving the winter. Unfortunately Japanese beetles seem to like the spathes.