Hello All: I'm with Diana on this one. I researched TMV and other tobamoviruses once, and even though I may not have the exact temperatures recalled correctly, it is virtually indestructible. It survives hours of boiling water, alcohol, and probably anything short of a blowtorch or a solution that would lyse it. If I remember, it can survive temps up to 400° F. If you're concerned about infection-- don't use old plant parts of infected plants., and sterilize cutting tools with Clorox, let 'em soak. Minimum dilution of 1:10 Clorox to water. Alcoholm won't do it.