Pollen Germination

Kathleen Sayce ksayce@willapabay.org
Sun, 21 Jul 2013 09:13:38 PDT
Short of sprouting pollen on agar, I don't have a good formula to check germination, but I do know about checking pollen viability:

Use the blue dye Cotton Blue. Put your pollen on a slide in water, add a drop off Cotton Blue (soluble in aqueous media), let it sit a few minutes, add a cover slip and check under the 'scope. 
The viable pollen will have taken up the dye and be blue, and the nonviable pollen will be colorless.


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