Dear Jan, Do you have any reference to using symbiotic embryo rescue in any Iris? I know of no study that suggest mycorrhizae are involved in germination of iris seeds. Jim W. On Nov 13, 2015, at 8:36 PM, Jan Jeddeloh <janjeddeloh@gmail.com> wrote: Has anyone tried doing embryo rescue with symbiotic rescue of the cultured embryo for wide iris crosses? This method works with lilies so I might be worth a try in iris. Jan Jeddeloh, Portland, Oregon James Waddick 8871 NW Brostrom Rd Kansas City, MO 64152-2711 USA Phone 816-746-1949