pbs Digest, Vol 50, Issue 2

Jonathan Knisely via pbs pbs@lists.pacificbulbsociety.net
Fri, 02 Apr 2021 05:12:26 PDT
>
> Re: Embryo Rescue




> What is the cause of the failure to develop endosperm in these
> intergeneric hybrids?
>
> Message: 1
> Date: Thu, 1 Apr 2021 17:00:51 +0000 (UTC)
> From: Robert Lauf <boblauf@att.net>
> To: Pacific Bulb Society <pbs@lists.pacificbulbsociety.net>
> Subject: [pbs] Embryo Rescue
> Message-ID: <176250100.3124430.1617296451883@mail.yahoo.com>
> Content-Type: text/plain; charset="utf-8"
>
> I recently made an intergeneric cross involving Hippeastrum and got a
> bunch of seeds that had little or no endosperm but otherwise looked ripe.?
> The seeds were surface-sterilized in hydrogen peroxide and placed in
> sterile culture medium.? A photo of the first baby is attached.? She says
> it looks like more are going to develop but they aren't as far along.
>
> A useful method that makes some wide crosses possible and is practically
> identical to the familiar techniques used for germinating orchid seeds.?
> With care, you can even do it at home in a little home-brew glovebox, altho
> this was done in a professional lab facility (laminar flow hood, etc.)?
> Yes, it's nice to have friends in high (or at least clean) places!
>
> Bob? Zone 7? after 7" of rain in the last week or so, now there's a
> threatened freeze tonight.? Spring in East Tenn...
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