clorox (and KMnO4)

Tim Eck via pbs pbs@lists.pacificbulbsociety.net
Thu, 07 Sep 2023 14:38:46 PDT
"Just remember that once you open the bottle, any unused solution will have
a limited shelf life."
I have to agree with this.  When I was sterilizing my magnolia cuttings, I
noticed my clorox didn't smell or look greenish yellow so I bought new.
(Long ago, I made the choice between functional elastic or bright white
underwear so my Clorox had been around for a while).
Check it out before you use it.  The laundry use is purely a learned
obsession but cutting sterilization is a must.
I wondered if they put detergent in the Clorox because it felt soapy but I
realized it would be saponifying the triglycerides in my skin because the
NaClO would be as caustic as NaOH since hypochlorous acid is so weak.
I suspect the outer cells of nearly everything are destroyed by NaClO but
us multicellular organisms are usually clad with disposable protective cells


On Thu, Sep 7, 2023 at 1:34 PM Robert Lauf via pbs <
pbs@lists.pacificbulbsociety.net> wrote:

>  If I were going to sterilize cuttings, I'd go with diluted clorox, but
> for more sensitive things, esp. embryo rescue, dipping them in hydrogen
> peroxide is usually sufficient to avoid contaminating the agar with mold
> while not killing the embryo.  It has the further advantage that any
> solution carried over into the flask decomposes fairly fast.  Typically the
> 3% antiseptic solution right out of the bottle from your favorite store is
> what is called for.  Just remember that once you open the bottle, any
> unused solution will have a limited shelf life.
> Bob  Zone 7 suffocating in the ragweed
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